Regulatory

Part:BBa_K1970003:Design

Designed by: Eivind Bøe Drejer   Group: iGEM16_NTNU_Trondheim   (2016-10-17)


Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 469
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designing recognition sequences that would bind to CI repressor mRNA with high affinity, but to the gene product of BBa_K1970002 with even greater affinity was very challenging.


Source

De novo DNA.

References